|
| Status |
Public on Aug 24, 2015 |
| Title |
HDF si-CSL_2 |
| Sample type |
SRA |
| |
|
| Source name |
HDF si-CSL
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: abdomen skin
cell type: primary dermal fibroblasts
passage: 5
transfected with: siRNA against CSL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the directZol RNA miniprep kit (Zymo Research) with on-column DNase treatment. RNA quality was verified on the bioanalyzer (Agilent) with RIN > 8
Four ug of total RNA were used for library preparation using the Truseq kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
MGP003
|
| Data processing |
trimming: Trimmomatic v.0.22
alignment: TopHat v.2.0.8b
expression levels: HTSeq package, release 0.5.4p1
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include gene expression values (expressed in count) for each Sample
|
| |
|
| Submission date |
Jan 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Paola Ostano |
| Organization name |
Fondazione Edo ed Elvo Tempia
|
| Street address |
via Malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE65473 |
Physical and functional CSL-p53 interactions underlie control of cancer stromal cell evolution [RNA-seq] |
| GSE65474 |
Physical and functional CSL-p53 interactions underlie control of cancer stromal cell evolution |
|
| Relations |
| BioSample |
SAMN03322718 |
| SRA |
SRX860956 |
