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Sample GSM1595925 Query DataSets for GSM1595925
Status Public on Jan 29, 2015
Title CD45-Ter119-Tie2-AlphaV+Thy-6C3-CD105+ rep3
Sample type RNA
 
Source name wild-type, anterior posterior limb
Organism Mus musculus
Characteristics age: postnatal day 3
tissue: fore and hind limbs
genotype: wild-type
cell population: CD45-Ter119-Tie2-AlphaV+Thy-6C3-CD105+
Growth protocol Skeletal tissues were dissected from P3 GFP-labeled mice and dissociated by mechanical and enzymatic dissociation. Specifically, the tissue was placed in collagenase digestion buffer supplemented with DNase and incubated at 37oC for 40 minutes under constant agitation. After collagenase digestion and neutralization, undigested materials were gently triturated by repeated pipetting. Total dissociated cells were filtered through 40 m nylon mesh, pelleted at 200g at 4oC, resuspended in staining media (2% fetal calf serum in PBS), blocked with rat IgG and stained with fluorochrome-conjugated antibodies against CD45, Tie2, AlphaV integrin, CD105, Thy1.1, Thy 1.2, 6C3 and CD200 for fractionation by fluorescence activated-cell sorting.
Extracted molecule total RNA
Extraction protocol RNA was isolated with RNeasy Micro Kit (Qiagen, Germantown, MD) as per manufacturer’s instructions. RNA was twice amplified with a RiboAmp RNA amplification kit (Arcturus Engineering, Mountain View, CA).
Label biotin
Label protocol Amplified cRNA was streptavidin-labeled, fragmented, and hybridized to Affymetrix 430-2.0 arrays as recommended by the manufacturer (Affymetrix, Santa Clara). Arrays were scanned with a Gene Chip Scanner 3000 (Affymetrix) running GCOS 1.1.1. software.
 
Hybridization protocol standard Affymetrix protocol
Scan protocol Arrays were scanned with a Gene Chip Scanner 3000 (Affymetrix) running GCOS 1.1.1. software.
Description fore and hind limbs pooled litter
Data processing Normalized Intenstity values were computed based on Gene Expression Commons Algorithum [ https://gexc.stanford.edu/]
 
Submission date Jan 29, 2015
Last update date Jan 29, 2015
Contact name Charles Chan KF
E-mail(s) chazchan@gmail.com
Organization name Stanford
Street address 265 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305-5461
Country USA
 
Platform ID GPL1261
Series (2)
GSE64447 Neonatal Skeletal Progenitors
GSE142874 Neonatal Skeletal Progenitors (Affymetrix)

Data table header descriptions
ID_REF
VALUE Normalized Intenstity values

Data table
ID_REF VALUE
1415670_at 10.32
1415671_at 10.36
1415672_at 10.73
1415673_at 7.29
1415674_a_at 8.44
1415675_at 8.96
1415676_a_at 11.46
1415677_at 8.66
1415678_at 10.27
1415679_at 10.66
1415680_at 9
1415681_at 8.52
1415682_at 7.07
1415683_at 9.67
1415684_at 7.49
1415685_at 7.94
1415686_at 8.18
1415687_a_at 11.12
1415688_at 9.72
1415689_s_at 8.34

Total number of rows: 45101

Table truncated, full table size 719 Kbytes.




Supplementary file Size Download File type/resource
GSM1595925_Charles_Chan_P3_BCSP-3_Mouse430_2_.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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