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Sample GSM1588603 Query DataSets for GSM1588603
Status Public on Jun 21, 2016
Title Atg7flox/flox;Nrf2flox/floxmice liver #3
Sample type RNA
Source name Atg7flox/flox;Nrf2flox/floxmice liver #3
Organism Mus musculus
Characteristics tissue: Liver
gender: female
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol (Life technologies, Carlsbad CA ) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol 200 ng of total RNA was amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit according to the manufacturer's protocol.
Hybridization protocol 1.65 µg of Cy3-labeled cRNA was fragmented and hybridized to Agilent Whole Mouse Genome Oligo Microarray (G4846A) using the Agilent Gene Expression Hybridization Kit. Hybridizations were performed for 17 hours at 65ºC at 10 rpm in a rotating hybridization oven.
Scan protocol Slides were scanned with SureScan Microarray Scanner (Agilent Technologies) using a scan resolution of 5µm and dye channel of Green with PMT set to 100%.
Description Gene expression of Atg7flox/flox;Nrf2flox/floxmice liver #3
Data processing Data was obtained using the Agilent Feature Extraction software (v11.5.1.1) with set to default for all parameters.
Submission date Jan 22, 2015
Last update date Jun 21, 2016
Contact name Tetsuya Saito
Phone 81-025-227-2080
Organization name Niigata University school of medicine
Department Biochemistry
Street address 757, Ichibancho, Asahimachidori, Chuo-ku
City Niigata-shi
State/province Niigata
ZIP/Postal code 951-8510
Country Japan
Platform ID GPL10333
Series (1)
GSE65174 Gene expressions in Atg7- and Atg7 Nrf2-deficient livers

Data table header descriptions
VALUE Normalized signal intensity (Shift to 75 percentile).

Data table
1 6.156159306
2 0.006926132
3 0.006976798
4 0.007022469
5 0.007063222
6 0.007100606
7 0.007132638
8 0.007161154
9 0.007185506
10 0.007205961
11 0.007222413
12 0.149820526
13 0.038539124
14 0.686373985
15 0.413078072
16 1.82991989
17 32.55015635
18 0.02279486
19 0.032671094
20 0.252250624

Total number of rows: 44397

Table truncated, full table size 764 Kbytes.

Supplementary file Size Download File type/resource
GSM1588603_SG12334215_252665516674_S001_GE1_1105_Oct12_1_1.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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