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Sample GSM1585605 Query DataSets for GSM1585605
Status Public on Dec 31, 2015
Title SOC-20, differentiated
Sample type RNA
Source name KT20-ad
Organism Homo sapiens
Characteristics cell type: differentiated ovarian cancer stem cells
Treatment protocol Differentiation of spheroid cells were induced by cultivating dissociated spheroid cells on standard culture dishes in the presence of 10% fetal bovine serum for 14 days.
Growth protocol Undifferentiated ovarian cancer stem cells were grown in grown in STEMPRO hESC SFM (Gibco) supplemented with 8 ng/ml bFGF (Invitrogen), penicillin/streptomycin, and in the presence or absence of 20 μM Y27632 (Wako) and/or 10 μg/ml insulin on ultra-low-attachment culture dishes (Corning).
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using miRNeasy mini kit (QIAGEN) according to the manufacturer’s protocol. RNA was quantified using a NanoDrop-1000 spectrophotometer. RNA quality control was performed in 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).
Label Cy3
Label protocol 100ng of total RNA was Cy3-labeled according to the manufacturer's protocol of On-Color Microarray-Based Gene Expression Analyses (Low Input Quick Amp Labeling), ver 6.5.
Hybridization protocol 0.6 ug of labelled cRNA was fd hybridized to Agilent Whole Human Genome Oligo Microarrays (G4851B, Agilent) for 17 hours at 65C in a rotating Agilent hybridization oven.
Scan protocol Slides were scanned on the Agilent DNA Microarray Scanner (Agilent).
Description Gene expression after differentiation
Data processing The scanned images were analyzed with Feature Extraction Software using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F/20120628).
Submission date Jan 15, 2015
Last update date Apr 23, 2018
Contact name koji okamoto
Phone 81335422511
Organization name National Cancer Center Research Institute
Department Division of Cancer Differentiation
Street address 5-1-1 Tsukiji
City Chuo-ku
State/province Tokyo
ZIP/Postal code 1040045
Country Japan
Platform ID GPL17077
Series (1)
GSE64999 Gene regulation during differentiation of ovarian cancer stem cells
Reanalyzed by GSE113533

Data table header descriptions
VALUE Normalized singla density

Data table
A_19_P00315452 0.792
A_19_P00315459 0.415
A_19_P00315482 0.029
A_19_P00315492 0.026
A_19_P00315493 0.698
A_19_P00315502 9.375
A_19_P00315506 2.656
A_19_P00315518 0.011
A_19_P00315519 0.006
A_19_P00315524 0.066
A_19_P00315528 0.008
A_19_P00315529 0.050
A_19_P00315538 0.075
A_19_P00315541 0.008
A_19_P00315543 0.030
A_19_P00315550 0.315
A_19_P00315551 0.387
A_19_P00315554 0.015
A_19_P00315581 6.063
A_19_P00315583 0.102

Total number of rows: 50683

Table truncated, full table size 970 Kbytes.

Supplementary file Size Download File type/resource
GSM1585605_US09503747_253949411824_S01_GE1_107_Sep09_2_4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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