GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1585600 Query DataSets for GSM1585600
Status Public on Dec 31, 2015
Title SOC-2, undifferentiated
Sample type RNA
Source name KT2-fl
Organism Homo sapiens
Characteristics cell type: undifferentiated ovarian cancer stem cells
Treatment protocol Differentiation of spheroid cells were induced by cultivating dissociated spheroid cells on standard culture dishes in the presence of 10% fetal bovine serum for 14 days.
Growth protocol Undifferentiated ovarian cancer stem cells were grown in grown in STEMPRO hESC SFM (Gibco) supplemented with 8 ng/ml bFGF (Invitrogen), penicillin/streptomycin, and in the presence or absence of 20 μM Y27632 (Wako) and/or 10 μg/ml insulin on ultra-low-attachment culture dishes (Corning).
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using miRNeasy mini kit (QIAGEN) according to the manufacturer’s protocol. RNA was quantified using a NanoDrop-1000 spectrophotometer. RNA quality control was performed in 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).
Label Cy3
Label protocol 100ng of total RNA was Cy3-labeled according to the manufacturer's protocol of On-Color Microarray-Based Gene Expression Analyses (Low Input Quick Amp Labeling), ver 6.5.
Hybridization protocol 0.6 ug of labelled cRNA was fd hybridized to Agilent Whole Human Genome Oligo Microarrays (G4851B, Agilent) for 17 hours at 65C in a rotating Agilent hybridization oven.
Scan protocol Slides were scanned on the Agilent DNA Microarray Scanner (Agilent).
Description Gene expression before differentiation
Data processing The scanned images were analyzed with Feature Extraction Software using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F/20120628).
Submission date Jan 15, 2015
Last update date Apr 23, 2018
Contact name koji okamoto
Phone 81335422511
Organization name National Cancer Center Research Institute
Department Division of Cancer Differentiation
Street address 5-1-1 Tsukiji
City Chuo-ku
State/province Tokyo
ZIP/Postal code 1040045
Country Japan
Platform ID GPL17077
Series (1)
GSE64999 Gene regulation during differentiation of ovarian cancer stem cells
Reanalyzed by GSE113533

Data table header descriptions
VALUE Normalized singla density

Data table
A_19_P00315452 0.038
A_19_P00315459 0.264
A_19_P00315482 0.103
A_19_P00315492 0.020
A_19_P00315493 0.508
A_19_P00315502 0.017
A_19_P00315506 1.199
A_19_P00315518 0.006
A_19_P00315519 0.006
A_19_P00315524 0.029
A_19_P00315528 0.214
A_19_P00315529 0.143
A_19_P00315538 0.006
A_19_P00315541 0.007
A_19_P00315543 0.028
A_19_P00315550 2.695
A_19_P00315551 1.657
A_19_P00315554 0.012
A_19_P00315581 8.119
A_19_P00315583 0.086

Total number of rows: 50683

Table truncated, full table size 970 Kbytes.

Supplementary file Size Download File type/resource
GSM1585600_US09503747_253949411824_S01_GE1_107_Sep09_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap