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Sample GSM1583303 Query DataSets for GSM1583303
Status Public on May 13, 2015
Title EML1 infected with the empty pWPI lentiviral vector treated with IL7 and FLT3L for 7 days- rep 1
Sample type RNA
Source name EML1 hematopoietic progenitor cell line
Organism Mus musculus
Characteristics cell line: EML1
treatment: empty pWPI lentiviral vector treated with IL7 and FLT3L for 7 days
Growth protocol EML1 cells were grown in IMDM (Iscove Modified Dulbecco’s Medium) supplemented with 20% heat-inactivated horse serum and 15% BHK/MKL cell-conditioned medium. For B-cell differentiation, OP9 stromal cells were plated in 6-well plates in Alpha Minimum Essential Medium (α-MEM) with 20% of fetal bovine serum. When they reached confluency, 50 000 EML1 cells were plated on top of the stromal cells in their specific culture medium plus supplements (IL7 10 ng/ml; FLT3L 80 ng/ml; β-mercaptoethanol, 50 μM).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA The size and the accuracy of quantitation of targets were checked by agarose gel electrophoresis of 2ug aliquots, prior to and after fragmentation. After fragmentation, targets were diluted in hybridisation buffer at a concentration of 150ug/ml.
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
Description EML1 cell line: Bone marrow cells of male BDF1 mice infected with the retroviral vector LRARalpha403SN. They can be induced with IL-7 and bone marrow stromal cells to express characteristics of pre-pro-B lymphocytes.
Data processing standard Affymetrix protocol
Submission date Jan 13, 2015
Last update date May 14, 2015
Contact name Myriam Alcalay
Organization name European Institute of Oncology
Department Experimental Oncology
Lab Functional Genomics
Street address Via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
Platform ID GPL11533
Series (1)
GSE64919 Genes regulated in EML1 cells expressing the TEL-AML1 oncogene after 5 and 7 days of treatment with IL7 and FLT3 ligand.

Data table header descriptions
VALUE normalized signal

Data table
10605884 53.2825
10411593 86.5031
10351644 609.767
10461553 48.1219
10436128 48.9233
10411595 395.284
10485979 44.2849
10545940 462.832
10521515 58.1219
10486081 56.5984
10595900 184.026
10485982 65.086
10606001 316.22
10461558 58.1058
10411609 448.823
10595892 54.6427
10571467 53.7783
10411611 331.145
10595895 55.8341
10546047 60.6662

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.

Supplementary file Size Download File type/resource
GSM1583303_EML-ev_B7_A.cel.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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