|
| Status |
Public on Jun 22, 2016 |
| Title |
HepG2 treated replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 cell line expressing HBV HBx protein
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
|
| Treatment protocol |
The cells were transduced with HBx-expressing or control adenovirus, treated with UVC (254nm) irradiation 48 h post-transduction and harvested 24 h after UV irradiation
|
| Growth protocol |
HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal calf serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen’s RNeasy mini kit according to manufacturer's protocol. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer .
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 100ng of total RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent, version 6.5) following manufacturer's intruction.
|
| |
|
| Hybridization protocol |
600ng of labeled cRNA was hybridized onto Agilent Sureprint G3 human GE 8x60K Microarray for 17 hours at 65oC, 10rpm in Agilent hybridization oven.
|
| Scan protocol |
After hybridization, the microarray slide was washed in gene expression wash buffer 1 for one minute at room temperature and another minute in gene epxression wash buffer 2 at 37oC before scanning on Agilent High Resolution Microarray Scanner (C-Model).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software v10.7.1.1 (Agilent) using default parameters.
|
| |
|
| Submission date |
Jan 12, 2015 |
| Last update date |
Jun 22, 2016 |
| Contact name |
Thomas Thurnherr |
| E-mail(s) |
thomas.thurnherr@gmail.com
|
| Organization name |
NCCS
|
| Lab |
LCFG
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13607 |
| Series (2) |
| GSE64875 |
Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein [Agilent] |
| GSE64878 |
Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein |
|
