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Sample GSM1581932 Query DataSets for GSM1581932
Status Public on Jun 22, 2016
Title HepG2 treated replicate 3
Sample type RNA
Source name HepG2 cell line expressing HBV HBx protein
Organism Homo sapiens
Characteristics cell line: HepG2
Treatment protocol The cells were transduced with HBx-expressing or control adenovirus, treated with UVC (254nm) irradiation 48 h post-transduction and harvested 24 h after UV irradiation
Growth protocol HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal calf serum.
Extracted molecule total RNA
Extraction protocol RNA was isolated using Qiagen’s RNeasy mini kit according to manufacturer's protocol. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer .
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 100ng of total RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent, version 6.5) following manufacturer's intruction.
Hybridization protocol 600ng of labeled cRNA was hybridized onto Agilent Sureprint G3 human GE 8x60K Microarray for 17 hours at 65oC, 10rpm in Agilent hybridization oven.
Scan protocol After hybridization, the microarray slide was washed in gene expression wash buffer 1 for one minute at room temperature and another minute in gene epxression wash buffer 2 at 37oC before scanning on Agilent High Resolution Microarray Scanner (C-Model).
Data processing The scanned images were analyzed with Feature Extraction Software v10.7.1.1 (Agilent) using default parameters.
Submission date Jan 12, 2015
Last update date Jun 22, 2016
Contact name Thomas Thurnherr
Organization name NCCS
Street address 11 Hospital Drive
City Singapore
ZIP/Postal code 169610
Country Singapore
Platform ID GPL13607
Series (2)
GSE64875 Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein [Agilent]
GSE64878 Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein

Data table header descriptions
VALUE Normalized signal intensity

Data table
4 9.057018599
5 12.98196764
6 8.214394912
7 14.23415042
8 11.51691829
9 6.40753296
10 7.266151895
11 6.678526477
12 10.80091252
13 9.916587017
14 10.42566938
15 15.37837284
16 6.237109933
17 8.498246624
18 6.154290269
19 6.398863303
20 11.58463714
21 11.10570051
24 8.301948533
25 10.71279805

Total number of rows: 42405

Table truncated, full table size 731 Kbytes.

Supplementary file Size Download File type/resource
GSM1581932_US83503546_252800414428_S01_GE1_107_Sep09_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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