|
| Status |
Public on Jan 09, 2015 |
| Title |
HuH7·IFN.rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HuH7_IFN
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HuH7
cell type: Human liver (hepato cellular carcinoma) cells
treated with: 10000 units/ml of IFN
time point: 3 days post-treatment
|
| Treatment protocol |
HuH7 cells were treated with 10000 units/ml of IFN a2 and RNA was isolated 3 days post-treatment
|
| Growth protocol |
HuH7 cells were incubated at 37 degress in 5% CO2 environment with DMEM media plus antibiotics and 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with TRIZOL (SIGMA)
|
| Label |
Cy3
|
| Label protocol |
For future sample normalization, RNA was treated with One-Color RNA Spike-In Kit cat. # 5188-5282 (Agilent). 200ng of RNA were used for cDNA preparation with Low Input Quick Amp Labeling Kit, one-color Cat. # 5190-2305, Agilent®. Sample labelling with dye3 (cy3) fluorofor was made with previous cited kit. Labelled cDNA was purified with RNeasy MinElute Cleanup Kit Cat.# 74204 (Izasa/Qiagen).
|
| |
|
| Hybridization protocol |
Labelled cDNA was purified with RNeasy MinElute Cleanup Kit Cat.# 74204 (Izasa/Qiagen). Labeled cDNA was hybridized to SurePrint G3 Human GE 8x60 Agilent® Microarray chip (ID: G4858A-028004).using Gene Expression Hybridization Kit Cat. # 5188-5242).
|
| Scan protocol |
Microarray Scanner Agilent G2505X class C
|
| Description |
Huh7_IFN_890_1_2_10NG893
|
| Data processing |
The data were normalized with Bioconductor software using quantiles.
|
| |
|
| Submission date |
Jan 08, 2015 |
| Last update date |
Jan 09, 2015 |
| Contact name |
Victor Segura |
| E-mail(s) |
vsegura@unav.es
|
| Organization name |
FIMA
|
| Department |
Unit of Proteomics, Genomics and Bioinformatics
|
| Street address |
Avda. Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13607 |
| Series (2) |
| GSE64794 |
Type I interferon regulates the expression of long non-coding RNAs [expression array] |
| GSE64796 |
Type I interferon regulates the expression of long non-coding RNAs |
|
