|
Status |
Public on Jan 09, 2015 |
Title |
HuH7·IFN.rep1 |
Sample type |
RNA |
|
|
Source name |
HuH7_IFN
|
Organism |
Homo sapiens |
Characteristics |
cell line: HuH7 cell type: Human liver (hepato cellular carcinoma) cells treated with: 10000 units/ml of IFN time point: 3 days post-treatment
|
Treatment protocol |
HuH7 cells were treated with 10000 units/ml of IFN a2 and RNA was isolated 3 days post-treatment
|
Growth protocol |
HuH7 cells were incubated at 37 degress in 5% CO2 environment with DMEM media plus antibiotics and 10% FCS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated with TRIZOL (SIGMA)
|
Label |
Cy3
|
Label protocol |
For future sample normalization, RNA was treated with One-Color RNA Spike-In Kit cat. # 5188-5282 (Agilent). 200ng of RNA were used for cDNA preparation with Low Input Quick Amp Labeling Kit, one-color Cat. # 5190-2305, Agilent®. Sample labelling with dye3 (cy3) fluorofor was made with previous cited kit. Labelled cDNA was purified with RNeasy MinElute Cleanup Kit Cat.# 74204 (Izasa/Qiagen).
|
|
|
Hybridization protocol |
Labelled cDNA was purified with RNeasy MinElute Cleanup Kit Cat.# 74204 (Izasa/Qiagen). Labeled cDNA was hybridized to SurePrint G3 Human GE 8x60 Agilent® Microarray chip (ID: G4858A-028004).using Gene Expression Hybridization Kit Cat. # 5188-5242).
|
Scan protocol |
Microarray Scanner Agilent G2505X class C
|
Description |
Huh7_IFN_890_1_4_10NG895
|
Data processing |
The data were normalized with Bioconductor software using quantiles.
|
|
|
Submission date |
Jan 08, 2015 |
Last update date |
Jan 09, 2015 |
Contact name |
Victor Segura |
E-mail(s) |
vsegura@unav.es
|
Organization name |
FIMA
|
Department |
Unit of Proteomics, Genomics and Bioinformatics
|
Street address |
Avda. Pío XII, 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL13607 |
Series (2) |
GSE64794 |
Type I interferon regulates the expression of long non-coding RNAs [expression array] |
GSE64796 |
Type I interferon regulates the expression of long non-coding RNAs |
|