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Sample GSM1574595 Query DataSets for GSM1574595
Status Public on Dec 31, 2014
Title Conventional WIS2 hESC (Fig. S7) rep1
Sample type SRA
 
Source name Human embryonic stem cell cultured in conventional condition
Organism Homo sapiens
Characteristics cell type: Human embryonic stem cell cultured in conventional condition
protocol: Unsorted
gender: Male
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using DirectZol RNA mini-prep. Libraries were prepared by the INCPM unit in the Weizmann Institute of Science according to Illumina's instructions accompanying the TruSeq RNA Sample Preparation Kit v2 (RS-122-2001).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Low quality bases (q < 20) from the 3' end and adapter sequences were removed with the TrimGalore wrapper scripts (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore).
Reads were mapped to the human (GRCh37/hg19) reference genome with Tophat 2.0.12 (http://ccb.jhu.edu/software/tophat)
Genome_build: GRCh37/hg19
Supplementary_files_format_and_content: Processed data files contain read counts per RefSeq transcripts generated by using the HTSeq 0.6.1 python package (http://www-huber.embl.de/users/anders/HTSeq)
 
Submission date Dec 30, 2014
Last update date May 15, 2019
Contact name Sabine Dietmann
Organization name Washington University School of Medicine
Street address 4565 McKinley Avenue
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL16791
Series (1)
GSE60138 SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Relations
Reanalyzed by GSE95736
BioSample SAMN03274001
SRA SRX825572

Supplementary file Size Download File type/resource
GSM1574595_esc.conv.1.counts.txt.gz 666.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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