|
Status |
Public on Jun 03, 2015 |
Title |
genomic DNA from bone of subject 48 |
Sample type |
genomic |
|
|
Source name |
Bone
|
Organism |
Homo sapiens |
Characteristics |
tissue: bone subject id: 48 Sex: female sexpredictedusingdnamethdata: female age: 82 dna methylation age: 81.8739021090779
|
Growth protocol |
DNA extracted from fresh frozen bone
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions. Further, we applied phenol–chloroform–isoamyl alcohol as described in Delgado-Calle J et al (2012) Epigenetics 2012; 7: 83–91. Pubmed ID: 23124911.
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
BeadStudio software v3.2. Unmethylated and methylated signal intensities and detection p values included in Series supplementary file
|
|
|
Submission date |
Dec 23, 2014 |
Last update date |
Jun 03, 2015 |
Contact name |
Steve Horvath |
E-mail(s) |
shorvath@altoslabs.com
|
Organization name |
University of California, Los Angeles
|
Department |
Human Genetics
|
Lab |
Horvath
|
Street address |
695 Charles E. Young Drive South, Box 708822
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-7088 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE64490 |
DNA methylation profiles of human bone samples |
GSE64511 |
DNA methylation profiles of multiple human tissues |
|