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Sample GSM1570973 Query DataSets for GSM1570973
Status Public on May 21, 2015
Title 8ep6_met
Sample type RNA
Source name Frozen ependymal tumor tissue
Organism Homo sapiens
Characteristics tissue: Ependymal tumor
primary/relapse: relapse
brain location: posterior fossa
age at diagnosis (years): 12
gender: f
molecular subgroup: PF_EPN_A
Treatment protocol Tumor samples were snap frozen upon resection and stored at -80 oC until DNA extraction. Tumor content was assessed from equivalent material fixed in formalin and used for histological diagnosis (all samples contained ≥70% tumor cells).
Extracted molecule total RNA
Extraction protocol Total RNA from tumour specimens was isolated after milling of the frozen sample in a Micro-Dismembrator S (B. Braun, Melsungen, Germany), using TRIzol (Invitrogen, Carlsbad, USA) and subsequent purification with a RNeasy Mini Spin Column (Qiagen, Hilden, Germany). Integrity and quantitation of total extracted RNA was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Wilmington, DE, USA).
Label Biotin
Label protocol Affymetrix protocol
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Description MAS5.0 normalized data
Data processing For normalization of the expression data we used the MAS5.0 algorithm of the GCOS program (Affymetrix Inc). Target intensity was set to 100 (α1= 0.04 and α2 = 0.06). Detection p-values were assigned to each probe set using the MAS5.0 algorithm (trimmed mean 96 = 100). Quality of the arrays was ensured by inspection of the ß-actin and GAPDH 5′-3′ ratios as well as the percentage of present calls generated by the MAS5.0 algorithm (Affymetrix Inc).
Submission date Dec 22, 2014
Last update date May 21, 2015
Contact name Marcel Kool
Organization name German Cancer Research Center DKFZ
Department Pediatric Neurooncology
Street address Im Neuenheimer Feld 280
City Heidelberg
ZIP/Postal code 69120
Country Germany
Platform ID GPL570
Series (1)
GSE64415 Gene expression data from ependymal tumor samples

Data table header descriptions
VALUE MAS5.0 signal intensity, log2 transformed

Data table
1552563_a_at 3.247927513
1552829_at 5.794415866
1552867_at 4.977279923
1552961_at 3
1552974_at 2.560714954
1552975_x_at 3.776103988
1552976_at 5.614709844
1553083_at 2.321928095
1553208_s_at 3.906890596
1553275_s_at 3.837943242
1553355_at 1.137503524
1553356_at 2.459431619
1553372_at 2.070389328
1553398_at 3.201633861
1553439_at 4.614709844
1553456_at 4.350497247
1553462_at 3.10433666
1553498_at 4.357552005
1553547_at 4.419538892
1553882_at 4.129283017

Total number of rows: 54675

Table truncated, full table size 1195 Kbytes.

Supplementary file Size Download File type/resource
GSM1570973_dkfz1588.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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