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Sample GSM157038 Query DataSets for GSM157038
Status Public on Feb 27, 2007
Title P_aeruginosa_rep2
Sample type RNA
 
Source name bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated
Organism Homo sapiens
Characteristics bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated, 4h, replicate 2
Extracted molecule total RNA
Extraction protocol RNA was isolated using HighPureTM RNA kit (Roche) as recommended by the manufacturer.
Label biotin
Label protocol Affymetrix one round amplification
 
Hybridization protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Scan protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Description Human bronchial epithelial cells BEAS-2B were stimulated in vitro with 10E8/ml
UV-inactivated P. aeruginosa or S. aureus or with RSV (MOI 1) for 4 h.
h.
Data processing Expression VALUES were generated using RMA (Robust Multi-Array Average expression measure) using R 2.4.0; Absolute Detection (ABS_CALL) was generated using the function mas5calls of the affy package for R.
 
Submission date Jan 19, 2007
Last update date Feb 27, 2007
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL571
Series (1)
GSE6802 Gene expression analysis of bronchial epithelial cells stimulated by different airway pathogens.

Data table header descriptions
ID_REF
VALUE linear expression value; rma
ABS_CALL MAS 5.0 absolute detection

Data table
ID_REF VALUE ABS_CALL
1007_s_at 586.94 P
1053_at 603.56 P
117_at 45.48 A
121_at 131.93 P
1255_g_at 11.72 A
1294_at 136.46 P
1316_at 42.99 A
1320_at 44.25 A
1405_i_at 17.1 P
1431_at 11.79 A
1438_at 46.45 A
1487_at 187.97 P
1494_f_at 28.66 A
1598_g_at 525.95 P
160020_at 136.26 P
1729_at 294.47 P
177_at 39.97 P
1773_at 88.18 P
179_at 303.48 A
1861_at 274.38 P

Total number of rows: 22277

Table truncated, full table size 421 Kbytes.




Supplementary file Size Download File type/resource
GSM157038.CEL.gz 2.0 Mb (ftp)(http) CEL

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