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Sample GSM157035 Query DataSets for GSM157035
Status Public on Feb 27, 2007
Title control_rep2
Sample type RNA
 
Source name bronchial epithelial cells BEAS-2B, not stimulated
Organism Homo sapiens
Characteristics bronchial epithelial cells BEAS-2B, not stimulated, 4h, replicate 2
Extracted molecule total RNA
Extraction protocol RNA was isolated using HighPureTM RNA kit (Roche) as recommended by the manufacturer.
Label biotin
Label protocol Affymetrix one round amplification
 
Hybridization protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Scan protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Description Human bronchial epithelial cells BEAS-2B were stimulated in vitro with 10E8/ml
UV-inactivated P. aeruginosa or S. aureus or with RSV (MOI 1) for 4 h.
Data processing Expression VALUES were generated using RMA (Robust Multi-Array Average expression measure) using R 2.4.0; Absolute Detection (ABS_CALL) was generated using the function mas5calls of the affy package for R.
 
Submission date Jan 19, 2007
Last update date Feb 27, 2007
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL571
Series (1)
GSE6802 Gene expression analysis of bronchial epithelial cells stimulated by different airway pathogens.

Data table header descriptions
ID_REF
VALUE linear expression value; rma
ABS_CALL MAS 5.0 absolute detection

Data table
ID_REF VALUE ABS_CALL
1007_s_at 541.55 P
1053_at 583.86 P
117_at 43.83 M
121_at 124.26 P
1255_g_at 12.44 A
1294_at 141.89 P
1316_at 37.68 A
1320_at 42.76 M
1405_i_at 17.03 P
1431_at 12.45 A
1438_at 36.49 A
1487_at 166.85 P
1494_f_at 26.7 M
1598_g_at 614.39 P
160020_at 185.03 P
1729_at 274.63 P
177_at 36.98 P
1773_at 97.95 P
179_at 295.75 A
1861_at 244.47 P

Total number of rows: 22277

Table truncated, full table size 421 Kbytes.




Supplementary file Size Download File type/resource
GSM157035.CEL.gz 1.8 Mb (ftp)(http) CEL

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