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Sample GSM1568383 Query DataSets for GSM1568383
Status Public on Jun 02, 2015
Title MCD_MY
Sample type RNA
 
Source name human urine sediments
Organism Homo sapiens
Characteristics sample type: urine sediments
gender: female
age: 26
ethnicity: Han
disease state: MCD
Treatment protocol [collection protocol] A whole-stream early-morning urine specimen was collected into sterile tubes before renal biopsy and processed within 3 hours.
Urine sample was centrifuged at 3,000 g for 30 min and at 13,000 g for 5 min at 4 °C. Urinary cell pellet was lysed by TRIzol® Reagent (Invitrogen™). Specimens were then stored at –80 °C until use.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit. This kit can be used to label any RNA sample, including total RNA, severely degraded RNA, plant RNA, and low molecular weight RNA.
 
Hybridization protocol The labled RNA were hybridized for 16 hr at 48C on Affymetrix miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description MicroRNA expression data from unrine sediments
Data processing The data were analyzed with miRNA QCTool using Affymetrix default analysis settings and quantile as normalization method.
 
Submission date Dec 18, 2014
Last update date Jun 02, 2015
Contact name Nannan Wang
E-mail(s) plumppumpkin@163.com
Organization name Chinese PLA General Hospital, Chinese PLA Institute of Nephrology,National Clinical Research Center for Kidney Diseases
Department Department of Nephrology
Lab State Key Laboratory of Kidney Diseases
Street address Fuxing Road NO.28
City Beijing
ZIP/Postal code 100853
Country China
 
Platform ID GPL19117
Series (1)
GSE64306 MicroRNA profiling by array of human urinary sediments from IgAN patients

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
MIMAT0000001_st 364.8544 P
MIMAT0015091_st 1.824668 A
MIMAT0000002_st 1.720287 A
MIMAT0015092_st 15.4721 P
MIMAT0020301_st 2.738326 P
MIMAT0000003_st 2.225528 A
MIMAT0020302_st 2.346291 P
MIMAT0000004_st 2.326053 A
MIMAT0000005_st 2.41804 P
MIMAT0015093_st 10.93809 P
MIMAT0020303_st 2.229856 A
MIMAT0000006_st 2.252619 A
MIMAT0020304_st 1.879215 A
MIMAT0000007_st 1.999405 A
MIMAT0015094_st 62.07669 P
MIMAT0000008_st 2.167146 A
MIMAT0020305_st 2.235058 A
MIMAT0000009_st 2.47076 P
MIMAT0020306_st 2.304034 A
MIMAT0000010_st 1.834095 A

Total number of rows: 36249

Table truncated, full table size 945 Kbytes.




Supplementary file Size Download File type/resource
GSM1568383_52MY.CEL.gz 831.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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