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Sample GSM156772 Query DataSets for GSM156772
Status Public on Mar 01, 2007
Title 20 MIN EGF STIMULATED MCF10A cells
Sample type RNA
 
Source name MCF-10A cells spontaneously immortalized normal human mammary epithelial cell line
Organism Homo sapiens
Characteristics MCF10A cells
Treatment protocol MCF10A cells were grown in DME:F12 medium supplemented with antibiotics, [10 mg/ml insulin, 0.1 mg/ml cholera toxin, 0.5 mg/ml hydrocortisone, heat-inactivated horse serum (5%); defined as 'serum' in the text] and 10ng/ml EGF. Cells were serum deprived for 24 hours.
Extracted molecule total RNA
Extraction protocol extraction of total RNA was performed using Versagene's RNA isolation kit according to the manufacture's protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U!33A2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using laser confocal scanner.
Description Gene expression data from 40 MIN EGF STIMULATED MCF10A cells.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Jan 18, 2007
Last update date Mar 01, 2007
Contact name Tal Shay
Organization name Weizmann Institute of Science
Department Physics of Complex Systems
Street address Herzel
City Rehovot
ZIP/Postal code 26100
Country Israel
 
Platform ID GPL571
Series (2)
GSE6784 Expression data from MCF10A cells subject to EGF stimulation
GSE6786 HeLa cells and MCF10A cells subject to EGF stimulation

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 184.3 P
AFFX-BioB-M_at 360.7 P
AFFX-BioB-3_at 205.9 P
AFFX-BioC-5_at 495 P
AFFX-BioC-3_at 669.3 P
AFFX-BioDn-5_at 1356.4 P
AFFX-BioDn-3_at 2165 P
AFFX-CreX-5_at 6153.1 P
AFFX-CreX-3_at 7446.2 P
AFFX-DapX-5_at 11.7 A
AFFX-DapX-M_at 1.9 A
AFFX-DapX-3_at 2.4 A
AFFX-LysX-5_at 10.9 A
AFFX-LysX-M_at 1 A
AFFX-LysX-3_at 11.4 A
AFFX-PheX-5_at 2.5 A
AFFX-PheX-M_at 0.8 A
AFFX-PheX-3_at 9.3 A
AFFX-ThrX-5_at 0.5 A
AFFX-ThrX-M_at 1.2 A

Total number of rows: 22277

Table truncated, full table size 388 Kbytes.




Supplementary file Size Download File type/resource
GSM156772.CEL.gz 3.1 Mb (ftp)(http) CEL

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