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Sample GSM156770 Query DataSets for GSM156770
Status Public on Mar 01, 2007
Title HELA cells stimulated with EGF for 480 minutes
Sample type RNA
 
Source name Human cervical carcinoma cell line
Organism Homo sapiens
Characteristics HeLa cells
Treatment protocol HELA cells were grown in DMEM supplemented with 10% FCS and antibiotics. Cells were serum deprived for 24 hours. Following stimulation with 10 ng/ml EGF for 480 minutes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U!33A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using laser confocal scanner.
Description Gene expression data from unstimulated HELA cells.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Jan 18, 2007
Last update date Mar 01, 2007
Contact name Tal Shay
Organization name Weizmann Institute of Science
Department Physics of Complex Systems
Street address Herzel
City Rehovot
ZIP/Postal code 26100
Country Israel
 
Platform ID GPL96
Series (2)
GSE6783 Expression data from HELA cells subject to EGF stimulation
GSE6786 HeLa cells and MCF10A cells subject to EGF stimulation

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 166.2 P
AFFX-BioB-M_at 403.6 P
AFFX-BioB-3_at 194.9 P
AFFX-BioC-5_at 517.4 P
AFFX-BioC-3_at 334.1 P
AFFX-BioDn-5_at 434.2 P
AFFX-BioDn-3_at 2923.9 P
AFFX-CreX-5_at 4171.8 P
AFFX-CreX-3_at 6286.2 P
AFFX-DapX-5_at 15.5 A
AFFX-DapX-M_at 16.7 A
AFFX-DapX-3_at 2.3 A
AFFX-LysX-5_at 1.4 A
AFFX-LysX-M_at 4.9 A
AFFX-LysX-3_at 11.7 A
AFFX-PheX-5_at 1.8 A
AFFX-PheX-M_at 0.8 A
AFFX-PheX-3_at 13.7 A
AFFX-ThrX-5_at 2.9 A
AFFX-ThrX-M_at 7.2 A

Total number of rows: 22283

Table truncated, full table size 389 Kbytes.




Supplementary file Size Download File type/resource
GSM156770.CEL.gz 3.4 Mb (ftp)(http) CEL

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