|
| Status |
Public on Dec 16, 2014 |
| Title |
Line 16 8 weeks |
| Sample type |
RNA |
| |
|
| Source name |
Human iPSC line 16 differentiated towards astrocytes for 8 weeks
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: in vitro cell culture
developmental lineage: Astroglial progenitor
differentiation age: 8 weeks of differentiation
hras status: HRAS-G12S mutant
|
| Treatment protocol |
Cells were dissociated with accutased and attached as a monolayer. Growth factors EGF and FGF2 were removed and replaced with CNTF.
|
| Growth protocol |
Maintained as a cell culture at 37C in 5% CO2 in neural media (DMEM/F12+glutamine, NEAA, N2, Heparin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by Rneasy and on-column DNAse digestion (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Performed at UCSF genomics core facility with standard procedures
|
| |
|
| Hybridization protocol |
Performed at UCSF genomics core facility with standard procedures
|
| Scan protocol |
Performed at UCSF genomics core facility with standard procedures
|
| Data processing |
Performed at UCSF genomics core facility with standard procedures
|
| |
|
| Submission date |
Dec 15, 2014 |
| Last update date |
Dec 16, 2014 |
| Contact name |
Robert Krencik |
| E-mail(s) |
rkrencik@houstonmethodist.org
|
| Phone |
7133639742
|
| Organization name |
Houston Methodist
|
| Department |
Neurosurgery
|
| Lab |
Krencik
|
| Street address |
6670 Bertner Ave.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE64194 |
Expression data to investigate Costello syndrome using human iPSCs differentiated into astroglial progenitors and astrocytes |
|
