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Sample GSM15648 Query DataSets for GSM15648
Status Public on Sep 17, 2004
Title Normal Megakaryocytes
Sample type RNA
 
Source name Bone marrow, TPO treated, CD34 posistive cells (6 donors pool)
Organism Homo sapiens
Extracted molecule total RNA
 
Description Normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; Genzyme, Boston, MA). Every 3 days, viable cells were scored by Trypan blue dye exclusion and cultures were amplified with fresh serum-free medium. Each well was then supplemented with 100 ng/mL TPO. After 14 to 16 days of liquid culture, CD34-derived megakaryocytes (MKs) were purified by means of an anti-CD41a monoclonal antibody (MoAb) (Dako, Milan, Italy) directed against the glycoproteic *IIb-*3 complex and immunobeads (MPC 450 Dynabeads; Dynal, Oslo, Norway), as previously described (27). The purity of MKs was determined for each isolation by indirect immunofluorescence, using an anti-CD41b MoAb which reacts with a different epitope of *IIb-*3 subunit (Immunotech Inc., Westbrook, ME), followed by a goat anti-mouse IgG, covalently linked to fluorescein (Becton Dickinson, San Jose, CA).
 
Submission date Jan 22, 2004
Last update date May 28, 2005
Contact name Elena Tenedini
E-mail(s) tenedini.elena@unimo.it
Phone 00390592055601
Fax 00390592055410
URL http://www.xlab.unimo.it
Organization name University of Modena and Reggio Emilia
Department department of biomedical sciences
Street address
City Modena
ZIP/Postal code 41100
Country Italy
 
Platform ID GPL96
Series (1)
GSE997 Essential Thrombocythemia megakaryocytes versus normal megakaryocytes

Data table header descriptions
ID_REF
VALUE Affymetrix MAS 5.0 Signal
ABS_CALL Affymetrix MAS 5.0 Detection

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 81.7 P
AFFX-BioB-M_at 161.5 P
AFFX-BioB-3_at 83.9 P
AFFX-BioC-5_at 290.3 P
AFFX-BioC-3_at 209.2 P
AFFX-BioDn-5_at 166.6 P
AFFX-BioDn-3_at 1321.7 P
AFFX-CreX-5_at 2149.9 P
AFFX-CreX-3_at 3257 P
AFFX-DapX-5_at 0.8 A
AFFX-DapX-M_at 2.9 A
AFFX-DapX-3_at 3 A
AFFX-LysX-5_at 5.2 A
AFFX-LysX-M_at 1.8 A
AFFX-LysX-3_at 0.4 A
AFFX-PheX-5_at 1.2 A
AFFX-PheX-M_at 1 A
AFFX-PheX-3_at 10.3 A
AFFX-ThrX-5_at 2.6 A
AFFX-ThrX-M_at 1.5 A

Total number of rows: 22283

Table truncated, full table size 384 Kbytes.




Supplementary data files not provided

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