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Status |
Public on Jul 31, 2015 |
Title |
Gas1 G6 |
Sample type |
RNA |
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|
Source name |
Mice developing DEN-induced hepatocellular carcinoma, subsequent transfection with Gas1
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Organism |
Mus musculus |
Characteristics |
strain: CD1 tissue: Liver gender: male age: ten week-old treatment: DEN-induced hepatocellular carcinoma, subsequent transfection with Gas1
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Treatment protocol |
Hydrodynamic gene delivery (HGD) was performed as described by Liu et al. {Liu, Song, et al. 1999 #16551}, using 50 μg of plasmid DNA.
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Growth protocol |
Ten week-old CD1 male mice were obtained from the animal facilities of the University of Valencia, and fed ad libitum with standard diet.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the TRIZOL method (Chomczynski and Sacchi, 1987)
|
Label |
Cy3
|
Label protocol |
100 ng of total RNA were labeled using LowInputQuick Amp Labeling kit (Agilent 5190-2305) following manufacturer instructions. Briefly: mRNA was reverse transcribed in the presence of T7-oligo-dT primer to produce cDNA, which was transcribed in vitro with T7 RNA polymerase in the presence of Cy3-CTP to produce labeled cRNA.
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Hybridization protocol |
The labeled cRNA was hybridized to the microarray according to the manufacturer's protocol.
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Scan protocol |
The arrays were washed, and scanned on an Agilent G2565CA microarray scanner at 100% PMT and 3 m resolution.
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Description |
Gene expression
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Data processing |
The intensity data was extracted using the Feature Extraction Software (Agilent).
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|
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Submission date |
Nov 24, 2014 |
Last update date |
Jul 31, 2015 |
Contact name |
Angela L. Riffo-Campos |
Organization name |
University of Valencia, and INCLIVA
|
Department |
Department of Biochemistry and Molecular Biology
|
Lab |
Epigenetics and Chromatin laboratory
|
Street address |
Avda. Blasco Ibáñes, 17
|
City |
Valencia |
State/province |
Valencia |
ZIP/Postal code |
46010 |
Country |
Spain |
|
|
Platform ID |
GPL13912 |
Series (1) |
GSE63574 |
Overexpression of the Growth arrest specific 1 (Gas1) gene reduces the in vivo progression of murine hepatocellular carcinoma and restores gene expression level |
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