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Sample GSM1552835 Query DataSets for GSM1552835
Status Public on Jul 31, 2015
Title Gas1 G6
Sample type RNA
 
Source name Mice developing DEN-induced hepatocellular carcinoma, subsequent transfection with Gas1
Organism Mus musculus
Characteristics strain: CD1
tissue: Liver
gender: male
age: ten week-old
treatment: DEN-induced hepatocellular carcinoma, subsequent transfection with Gas1
Treatment protocol Hydrodynamic gene delivery (HGD) was performed as described by Liu et al. {Liu, Song, et al. 1999 #16551}, using 50 μg of plasmid DNA.
Growth protocol Ten week-old CD1 male mice were obtained from the animal facilities of the University of Valencia, and fed ad libitum with standard diet.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the TRIZOL method (Chomczynski and Sacchi, 1987)
Label Cy3
Label protocol 100 ng of total RNA were labeled using LowInputQuick Amp Labeling kit (Agilent 5190-2305) following manufacturer instructions. Briefly: mRNA was reverse transcribed in the presence of T7-oligo-dT primer to produce cDNA, which was transcribed in vitro with T7 RNA polymerase in the presence of Cy3-CTP to produce labeled cRNA.
 
Hybridization protocol The labeled cRNA was hybridized to the microarray according to the manufacturer's protocol.
Scan protocol The arrays were washed, and scanned on an Agilent G2565CA microarray scanner at 100% PMT and 3 m resolution.
Description Gene expression
Data processing The intensity data was extracted using the Feature Extraction Software (Agilent).
 
Submission date Nov 24, 2014
Last update date Jul 31, 2015
Contact name Angela L. Riffo-Campos
Organization name University of Valencia, and INCLIVA
Department Department of Biochemistry and Molecular Biology
Lab Epigenetics and Chromatin laboratory
Street address Avda. Blasco Ibáñes, 17
City Valencia
State/province Valencia
ZIP/Postal code 46010
Country Spain
 
Platform ID GPL13912
Series (1)
GSE63574 Overexpression of the Growth arrest specific 1 (Gas1) gene reduces the in vivo progression of murine hepatocellular carcinoma and restores gene expression level

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 11.06
2 5.80
3 5.88
4 5.92
5 6.17
6 6.05
7 5.94
8 7.90
9 5.91
10 6.17
11 6.15
12 5.87
13 6.19
14 9.96
15 6.28
16 5.94
17 8.08
18 6.10
19 7.10
20 12.44

Total number of rows: 62976

Table truncated, full table size 669 Kbytes.




Supplementary file Size Download File type/resource
GSM1552835_01_IG_1100252800513861_Cy3_R12_1_1.txt.gz 11.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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