|
| Status |
Public on Jul 31, 2015 |
| Title |
Control C2 |
| Sample type |
RNA |
| |
|
| Source name |
control
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
tissue: Liver
gender: male
age: ten week-old
treatment: control
|
| Treatment protocol |
Hydrodynamic gene delivery (HGD) was performed as described by Liu et al. {Liu, Song, et al. 1999 #16551}, using 50 μg of plasmid DNA.
|
| Growth protocol |
Ten week-old CD1 male mice were obtained from the animal facilities of the University of Valencia, and fed ad libitum with standard diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the TRIZOL method (Chomczynski and Sacchi, 1987)
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA were labeled using LowInputQuick Amp Labeling kit (Agilent 5190-2305) following manufacturer instructions. Briefly: mRNA was reverse transcribed in the presence of T7-oligo-dT primer to produce cDNA, which was transcribed in vitro with T7 RNA polymerase in the presence of Cy3-CTP to produce labeled cRNA.
|
| |
|
| Hybridization protocol |
The labeled cRNA was hybridized to the microarray according to the manufacturer's protocol.
|
| Scan protocol |
The arrays were washed, and scanned on an Agilent G2565CA microarray scanner at 100% PMT and 3 m resolution.
|
| Description |
Gene expression
|
| Data processing |
The intensity data was extracted using the Feature Extraction Software (Agilent).
|
| |
|
| Submission date |
Nov 24, 2014 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Angela L. Riffo-Campos |
| Organization name |
University of Valencia, and INCLIVA
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Epigenetics and Chromatin laboratory
|
| Street address |
Avda. Blasco Ibáñes, 17
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE63574 |
Overexpression of the Growth arrest specific 1 (Gas1) gene reduces the in vivo progression of murine hepatocellular carcinoma and restores gene expression level |
|
