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Sample GSM1551885 Query DataSets for GSM1551885
Status Public on Jul 25, 2016
Title HeLa-S3_LATS2_KO
Sample type RNA
Source name HeLa-S3 cell, LATS2 KO, clone#25
Organism Homo sapiens
Characteristics cell line: HeLa-S3
tissue: cervix
genotype: LATS2-/-
Treatment protocol no treatment
Growth protocol LATS2 KO HeLa-S3 cells and parental wild type cells were cultured in DMEM + 10% fetal bovine serum (FBS), penicillin (100U/mL), and streptomycin (100μg/mL) at 37°C, 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cultured cells with miRNeasy Mini Kit (Qiagen) and processed according to the manufacturer’s protocol. The quality of the RNAs was estimated by using the RNA 6000 Nano LabChip Kit (p/n 5065-4476) on the Agilent 2100 Bioanalyzer (G2940BA; Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol 100 ng of total RNAs were independently reverse-transcribed using oligo-dT primers containing the T7 RNA polymerase promoter sequence to generate cDNAs and AffinityScript, RTase, which were then subjected to in vitro transcription using T7 RNA polymerase to label the cRNAs with Cy3-CTP (Amersham Pharmacia Biotech, Piscataway, NJ) using a Low input Quick-Amp Labeling Kit (Agilent Technologies).
Hybridization protocol Before hybridization, 1650 ng labeled cRNA of each product was fragmented and mixed with control targets and hybridization buffer according to the manufacturer's protocol (Agilent Technologies). Hybridizations to Agilent-014850 arrays were done for approximately 17h at 65°C. The slides were washed according to the manufacturer's manual.
Scan protocol Scanning of microarrays was performed with 5µm resolution using a DNA microarray laser scanner (Agilent Technologies). The array was scanned using Agilent G2505C DNA microarray scanner. Images were quantified using Agilent Feature Extraction Software (version
Description HeLa-S3 cell, LATS2 KO, clone#25
Data processing Agilent Feature Extraction Software (v was used for background subtraction, LOWESS normalization, and dye-normalization.
Submission date Nov 21, 2014
Last update date Jul 25, 2016
Contact name Daisuke Okuzaki
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platform ID GPL13607
Series (2)
GSE63534 Transcriptome profiling of TALEN-mediated LATS2 knockout HeLa-S3 cells
GSE63538 LATS2 regulates repressive epigenetic integrity via regulation of Polycomb repressive complex 2

Data table header descriptions
VALUE Processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
1 2.90E+04
2 7.88E+00
3 7.91E+00
4 6.99E+02
5 4.85E+03
6 5.35E+01
7 2.04E+04
8 7.35E+02
9 8.03E+00
10 9.84E+01
11 8.05E+00
12 1.90E+03
13 8.63E+02
14 3.58E+02
15 4.76E+04
16 8.08E+00
17 2.48E+02
18 8.07E+00
19 2.26E+01
20 2.09E+03

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM1551885_HeLa-S3_LATS2KO_25_2_3.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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