|
Status |
Public on Mar 01, 2015 |
Title |
Ago2 IP miR-191 BioRep1_chip |
Sample type |
RNA |
|
|
Source name |
HeLa cell_transfected with miR-191 duplex
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa antibody: Ago2 (Abcam ab57113)
|
Treatment protocol |
mature miR-191 duplex at a concentration of 100nM was transfected with lipofectamine 2000
|
Growth protocol |
DMEM supplemented with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with TRIzol reagent
|
Label |
Cy3
|
Label protocol |
Standard Illumina TotalPrep RNA Amplification Kit protocol
|
|
|
Hybridization protocol |
Standard Illumina TotalPrep RNA Amplification Kit protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
RNA isolated from Ago2 immunoprecipitation
|
Data processing |
Quantile normalized using Illumina BeadStudio
|
|
|
Submission date |
Nov 19, 2014 |
Last update date |
Mar 01, 2015 |
Contact name |
Vishy Iyer |
E-mail(s) |
iyerlab@gmail.com
|
Phone |
5122327833
|
Organization name |
University of Texas at Austin
|
Department |
Molecular Biosciences
|
Street address |
2500 Speedway Dr. MBB 3.212
|
City |
Austin |
State/province |
TX |
ZIP/Postal code |
78712 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE63483 |
Genome-wide hsa-miR-191 target profile [chip] |
GSE63556 |
Genome wide miR-191 target profile determined by RIP and gene expression profiling |
|