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Sample GSM1547790 Query DataSets for GSM1547790
Status Public on Nov 30, 2015
Title shRNANIK3
Sample type RNA
 
Source name MCF7 cell line
Organism Homo sapiens
Characteristics cell line: MCF7
sample type: NIK inhibited
Treatment protocol Only the transfection
Growth protocol MCF7 cells were transiently transfected with a shRNA-NIK using lipofectamine 2000 (invitrogen) . A shRNA directed against luciferase (siRNA-Luc) was employed as a control. In addition, to overexpress NIK, MCF7 cells were transiently transfected with an expression vector (pt-REX-NIK), which contains the complete ORF of NIK. . The cells were analyzed for NIK expression 48 hours after transfection
Extracted molecule total RNA
Extraction protocol RNA Extraction with Trizol
Label non
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Gene expression data of MCF7shRNANIK3
Data processing Raw CEL data were normalized using the Robust Multi-array Average (RMA) method, as implemented in the oligo package . The Limma R package was used for differential expression analysis . We identified genes with a >2-fold change and a crude p<0.05.
 
Submission date Nov 17, 2014
Last update date Nov 30, 2015
Contact name Vilma Araceli Maldonado
E-mail(s) vmaldonado@inmegen.gob.mx
Phone 5553501900
Organization name INMEGEN
Department Investigacion
Lab Number 3
Street address Periferico 4809
City México
State/province Distrito Federal
ZIP/Postal code 14610
Country Mexico
 
Platform ID GPL10739
Series (1)
GSE63382 Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 6.38761
7892502 6.23206
7892503 6.16699
7892504 10.32445
7892505 4.43217
7892506 6.71345
7892507 7.38326
7892508 9.52505
7892509 12.81933
7892510 5.50282
7892511 5.73782
7892512 8.49172
7892513 3.59995
7892514 12.41119
7892515 10.55963
7892516 7.09248
7892517 7.51671
7892518 5.17708
7892519 6.53511
7892520 10.48888

Total number of rows: 65528

Table truncated, full table size 1029 Kbytes.




Supplementary file Size Download File type/resource
GSM1547790_KVS006.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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