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Sample GSM1547786 Query DataSets for GSM1547786
Status Public on Nov 30, 2015
Title shRNALuc2
Sample type RNA
Source name MCF7 cell line
Organism Homo sapiens
Characteristics cell line: MCF7
sample type: Vector control
Treatment protocol Only the transfection
Growth protocol MCF7 cells were transiently transfected with a shRNA-NIK using lipofectamine 2000 (invitrogen) . A shRNA directed against luciferase (siRNA-Luc) was employed as a control. In addition, to overexpress NIK, MCF7 cells were transiently transfected with an expression vector (pt-REX-NIK), which contains the complete ORF of NIK. . The cells were analyzed for NIK expression 48 hours after transfection
Extracted molecule total RNA
Extraction protocol RNA Extraction with Trizol
Label non
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Gene expression data of MCF7shRNALUC2
Data processing Raw CEL data were normalized using the Robust Multi-array Average (RMA) method, as implemented in the oligo package . The Limma R package was used for differential expression analysis . We identified genes with a >2-fold change and a crude p<0.05.
Submission date Nov 17, 2014
Last update date Nov 30, 2015
Contact name Vilma Araceli Maldonado
Phone 5553501900
Organization name INMEGEN
Department Investigacion
Lab Number 3
Street address Periferico 4809
City México
State/province Distrito Federal
ZIP/Postal code 14610
Country Mexico
Platform ID GPL10739
Series (1)
GSE63382 Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells

Data table header descriptions

Data table
7892501 5.91194
7892502 6.09504
7892503 4.14965
7892504 10.19439
7892505 3.67855
7892506 5.79717
7892507 7.23346
7892508 8.70017
7892509 12.53539
7892510 5.10198
7892511 5.64078
7892512 9.0372
7892513 3.53633
7892514 12.29001
7892515 10.11394
7892516 6.14253
7892517 6.6844
7892518 4.02217
7892519 6.96904
7892520 10.13203

Total number of rows: 65528

Table truncated, full table size 1028 Kbytes.

Supplementary file Size Download File type/resource
GSM1547786_KVS002.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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