NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1547785 Query DataSets for GSM1547785
Status Public on Nov 30, 2015
Title shRNALuc1
Sample type RNA
 
Source name MCF7 cell line
Organism Homo sapiens
Characteristics cell line: MCF7
sample type: Vector control
Treatment protocol Only the transfection
Growth protocol MCF7 cells were transiently transfected with a shRNA-NIK using lipofectamine 2000 (invitrogen) . A shRNA directed against luciferase (siRNA-Luc) was employed as a control. In addition, to overexpress NIK, MCF7 cells were transiently transfected with an expression vector (pt-REX-NIK), which contains the complete ORF of NIK. . The cells were analyzed for NIK expression 48 hours after transfection
Extracted molecule total RNA
Extraction protocol RNA Extraction with Trizol
Label non
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Gene expression data of MCF7shRNALUC1
Data processing Raw CEL data were normalized using the Robust Multi-array Average (RMA) method, as implemented in the oligo package . The Limma R package was used for differential expression analysis . We identified genes with a >2-fold change and a crude p<0.05.
 
Submission date Nov 17, 2014
Last update date Nov 30, 2015
Contact name Vilma Araceli Maldonado
E-mail(s) vmaldonado@inmegen.gob.mx
Phone 5553501900
Organization name INMEGEN
Department Investigacion
Lab Number 3
Street address Periferico 4809
City México
State/province Distrito Federal
ZIP/Postal code 14610
Country Mexico
 
Platform ID GPL10739
Series (1)
GSE63382 Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 5.75849
7892502 6.32283
7892503 5.04483
7892504 10.17436
7892505 4.0976
7892506 5.7936
7892507 7.51044
7892508 9.03769
7892509 12.57438
7892510 5.31476
7892511 5.08622
7892512 9.05628
7892513 4.39903
7892514 12.45794
7892515 10.43256
7892516 6.80833
7892517 7.14548
7892518 4.79911
7892519 6.59264
7892520 10.44317

Total number of rows: 65528

Table truncated, full table size 1028 Kbytes.




Supplementary file Size Download File type/resource
GSM1547785_KVS001.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap