|
| Status |
Public on Mar 08, 2007 |
| Title |
23-29 nucleotide RNAs from Drosophila melanogaster ovaries |
| Sample type |
SRA |
| |
|
| Source name |
Hannon lab, Cold Spring Harbor Labs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
small RNAs (23-29nt) from total ovarian RNA, indicating the cloning frequency
|
| Extracted molecule |
total RNA |
| Extraction protocol |
small RNA was isolated from dissected ovaries using TRIZOL and size separated on a 15% denaturing polyacrylamid gel.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
cDNA library was prepared according to standard small RNA cloning procedures (Pfeffer S. et al. 2005; Nat. Methods)
|
| Data processing |
library was sequenced at 454 Life Sciences and small RNA were extracted bioinformatically as the sequence flanked by the two cloning linkers.
|
| |
|
| Submission date |
Jan 10, 2007 |
| Last update date |
May 28, 2014 |
| Contact name |
Julius Brennecke |
| E-mail(s) |
brenneck@cshl.edu
|
| Organization name |
Cold Spring Harbor Labs
|
| Lab |
Greg Hannon
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL9058 |
| Series (1) |
| GSE6734 |
piRNA libraries from Drosophila melanogaster ovaries |
|
| Relations |
| BioSample |
SAMN02195376 |
