|
| Status |
Public on Apr 02, 2015 |
| Title |
Epidermis_psoriasis_PRE-UV_2 |
| Sample type |
genomic |
| |
|
| Source name |
psoriatic epidermis before phototherapy patient 2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
treatment: PRE-UV
tissue: psoriatic epidermis before phototherapy
|
| Treatment protocol |
Skin biopsies were incubated in 3.8% ammonium thiocyanate at room temperature for 20 min for separation of epidermis from dermis
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from epidermis using PureLink® Genomic DNA Kits from Life Technologies
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium HumanMethylation450 BeadChip using standard Illumina protocol
|
| Scan protocol |
iScan Reader was used to image BeadChips
|
| Description |
psoriasis
SAMPLE 2
|
| Data processing |
ChAMP pipeline (probe filtering, BMIQ normalization)
|
| |
|
| Submission date |
Nov 14, 2014 |
| Last update date |
Apr 02, 2015 |
| Contact name |
Xiaolian Gu |
| Organization name |
Umeå University
|
| Department |
Medical Biosciences
|
| Lab |
Pathology
|
| Street address |
Building 6M, 2nd floor, Umeå University
|
| City |
Umeå |
| State/province |
västerbotton |
| ZIP/Postal code |
90187 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE63315 |
Narrow-band UVB phototherapy reverses DNA methylation changes in psoriatic epidermis |
|
