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Sample GSM1545155 Query DataSets for GSM1545155
Status Public on Mar 21, 2016
Title Wapal shRNA2 Rep 2
Sample type RNA
Source name Wapal shRNA2
Organism Mus musculus
Characteristics cell type: ESC
genotype/variation: Wapal shRNA2
Treatment protocol Adherent cells were infected with lentivirus for 48 hrs in the presence of polybrene 10 mcg/mL. Media was then changed to ES cell media with LIF and puromycin (4 mcg/mL). Cells were then cultured for 48hrs post infection.
Growth protocol CJ9 (129svj derived ESCs) were grown under standard conditions prior to infection
Extracted molecule total RNA
Extraction protocol Cells were lysed with Trizol and total RNA collected. Total RNA was further purified by the Qiagen RNA isolation system per manufacturer's protocol.
Label Biotin
Label protocol The cDNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual
Hybridization protocol Per affymetrix manual
Scan protocol After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner LR15
Data processing .gct and .res files were created by RMA normalization using GenePattern ( These files (specifically the .gct) was used for downstream analysis by GSEA
Submission date Nov 14, 2014
Last update date Mar 22, 2016
Contact name Sridhar Rao
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
Platform ID GPL1261
Series (2)
GSE63291 The cohesin offloading factor Wapal is required for proper polycomb-mediated gene silencing [array]
GSE63325 The cohesin associated factor Wapal is required for proper polycomb-mediated gene silencing

Data table header descriptions
VALUE RMA normalized intensities

Data table
1415670_at 295.8663892
1415671_at 1412.888564
1415672_at 2599.421945
1415673_at 856.4443024
1415674_a_at 892.1423
1415675_at 366.4734102
1415676_a_at 4650.873692
1415677_at 193.9887212
1415678_at 1185.916202
1415679_at 1704.69526
1415680_at 710.5526747
1415681_at 1528.535368
1415682_at 212.6178137
1415683_at 3634.536839
1415684_at 423.6231475
1415685_at 989.2523594
1415686_at 401.8047207
1415687_a_at 543.35786
1415688_at 2087.876589
1415689_s_at 362.6202482

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM1545155_wapal_shRNA2_2.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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