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Sample GSM1538574 Query DataSets for GSM1538574
Status Public on Feb 09, 2017
Title CHC001 PDX NT - replicate 1
Sample type RNA
 
Source name CHC001 PDX NT - replicate 1
Organism Homo sapiens
Characteristics xenograft source: patient-derived CHC001 PDX
treatment: PBS
Treatment protocol EGI-1 xenografts and CHC001 PDXs were treated with 0.15 mg/Kg weekly i.v. (three administrations) or with PBS alone
Growth protocol RPMI + 10%FBS
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions. Absolutely RNA miRNA Kit
Label Cy3
Label protocol 100 ng of mRNA was amplified and labeled using One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling kit (Agilent)
 
Hybridization protocol 600 ng of labeled samples were hybridized on 8 x 60K glass array; after 17 hours of incubation at 65°C, the slide was washed following the manufacturers' instructions.
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 9.5).
Data processing Raw data were background corrected (normexp method) and quantile normalized using bioconductor.
 
Submission date Nov 06, 2014
Last update date Apr 23, 2018
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL17077
Series (1)
GSE63043 Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE normalized log2 data

Data table
ID_REF VALUE
A_23_P117082 11.6918019
A_33_P3246448 6.866144375
A_33_P3318220 6.49491377
A_33_P3236322 6.815405485
A_33_P3319925 7.881702797
A_21_P0000509 15.14682742
A_21_P0000744 9.657939981
A_24_P215804 6.528856275
A_23_P110167 13.47517437
A_33_P3211513 9.899816925
A_23_P103349 6.512311779
A_32_P61480 6.686249351
A_33_P3788124 6.409698639
A_33_P3414202 10.89245681
A_33_P3316686 10.45339371
A_33_P3300975 10.43441076
A_33_P3263061 12.54483928
A_33_P3261373 6.627697809
A_24_P278460 10.23587021
A_21_P0013109 6.409698639

Total number of rows: 50599

Table truncated, full table size 1255 Kbytes.




Supplementary file Size Download File type/resource
GSM1538574_US22502614_253949410796_S01_GE1_107_Sep09_2_NO_SPIKEIN_1_1.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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