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Sample GSM1536673 Query DataSets for GSM1536673
Status Public on Dec 30, 2014
Title LW11
Sample type SRA
Source name iPSC-derived neuron sample
Organism Homo sapiens
Characteristics age: ~ Differentiation day 45
disease: Healthy
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy Micro Kit (QIAGEN). Quality control of the RNA was carried out with the Agilent Bio-analyzer, Qubit 2.0 at the MPSR of Columbia University. 100 ng of RNA with RIN ≥ 9 were used for generating mRNA-focused libraries.
RNA libraries were prepared using TruSeq RNA Sample Preparation Kit v2
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description C2
Data processing Hierarchical Clustering was done in Cluster (de Hoon et al., 2004) and visualized in Treeview (Saldanha, 2004). For each experiment and every gene, the gene expression values in FPKM was median centered and normalized to have unit variance before clustering. One matrix CIM (http: //discover.nci. is used to generate the cluster heatmap.
Supplementary_files_format_and_content: csv files include FPKM values for each Sample
Submission date Nov 03, 2014
Last update date May 15, 2019
Contact name Liheng Wang
Organization name Columbia University Medical Center
Department Department of Medicine
Lab Domenico Accili
Street address 1150 Saint Nicholas Avenue, Room 234
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platform ID GPL16791
Series (1)
GSE62936 RNA-Seq Analysis in hES/ iPS cell-derived neuronal samples
BioSample SAMN03160331
SRA SRX750249

Supplementary file Size Download File type/resource
GSM1536673_LW11_genes.csv.gz 941.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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