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Sample GSM1536669 Query DataSets for GSM1536669
Status Public on Dec 30, 2014
Title LW06
Sample type SRA
 
Source name NKX2.1 GFP/W-hESC-derived neurons
Organism Homo sapiens
Characteristics age: ~ Differentiation day 27
disease: Healthy
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy Micro Kit (QIAGEN). Quality control of the RNA was carried out with the Agilent Bio-analyzer, Qubit 2.0 at the MPSR of Columbia University. 100 ng of RNA with RIN ≥ 9 were used for generating mRNA-focused libraries.
RNA libraries were prepared using TruSeq RNA Sample Preparation Kit v2
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Day 27 neurons
Data processing Hierarchical Clustering was done in Cluster (de Hoon et al., 2004) and visualized in Treeview (Saldanha, 2004). For each experiment and every gene, the gene expression values in FPKM was median centered and normalized to have unit variance before clustering. One matrix CIM (http: //discover.nci. nih.gov/cimminer/home.do) is used to generate the cluster heatmap.
Supplementary_files_format_and_content: csv files include FPKM values for each Sample
 
Submission date Nov 03, 2014
Last update date May 15, 2019
Contact name Liheng Wang
E-mail(s) lw2381@cumc.columbia.edu
Organization name Columbia University Medical Center
Department Department of Medicine
Lab Domenico Accili
Street address 1150 Saint Nicholas Avenue, Room 234
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL16791
Series (1)
GSE62936 RNA-Seq Analysis in hES/ iPS cell-derived neuronal samples
Relations
BioSample SAMN03160341
SRA SRX750245

Supplementary file Size Download File type/resource
GSM1536669_LW06_genes.csv.gz 941.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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