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Sample GSM1531906 Query DataSets for GSM1531906
Status Public on May 25, 2015
Title GF2
Sample type SRA
 
Source name Brain
Organism Mus musculus
Characteristics cell type: Microglia
strain: C57BL/6 (germ-free)
age: 6-8 weeks
Treatment protocol no prior treatment
Growth protocol Mice were housed under SPF or germ-free condiotions. Animals were 6-8 weeks old.
Extracted molecule total RNA
Extraction protocol Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer.
3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description polyA+ RNA
Data processing Illumina Casava1.8 software used for basecalling.
alignment: tophat with deafult parameters
Expression table done using ESAT http://garberlab.umassmed.edu/software/esat/
Genome_build: mm9
Supplementary_files_format_and_content: tab delimited
 
Submission date Oct 24, 2014
Last update date May 15, 2019
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platform ID GPL18480
Series (1)
GSE62710 Commensal microbiota constantly control maturation and function of microglia in the central nervous system
Relations
BioSample SAMN03143011
SRA SRX743394

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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