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Sample GSM1531748 Query DataSets for GSM1531748
Status Public on Dec 11, 2014
Title moDC_sh-Cebpb H3K4me1
Sample type SRA
Source name MC_A10_cDC_sh-Cebpb_K4me1
Organism Mus musculus
Characteristics cell type: moDC
tissue: Bone marrow
strain: C57BL/6
chip antibody: H3K4me1
Growth protocol Bone marrows were extracted from femora and tibiae of C57BL/6J female mice (6 to 10 weeks old), growth in presence of GM-CSF (for moDC) or Flt3L (for pDC).
Extracted molecule genomic DNA
Extraction protocol Cells were fixated, lyzed and nuclei were sonicated. Sonicated nuclei were mixed with protein G magnetic dynabeads coupled to target antibbody.
Libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
Data processing Basecalls performed using CASAVA version 1.4
alignment using bowtie 0.12 using default parameters
tdf command - igvtools count -w 5 -e 200
Genome_build: mm9
Supplementary_files_format_and_content: tdf
Submission date Oct 24, 2014
Last update date May 15, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platform ID GPL18480
Series (1)
GSE62702 A negative feedback loop of transcription factors specifies alternative dendritic cell chromatin states (ChIP-Seq)
BioSample SAMN03142920
SRA SRX742836

Supplementary file Size Download File type/resource
GSM1531748_MC_A10_cDC_sh-Cebpb_K4me1.tdf 346.8 Mb (ftp)(http) TDF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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