|
Status |
Public on Dec 03, 2014 |
Title |
mon_day7-1 |
Sample type |
SRA |
|
|
Source name |
Monocyte cells sorted from spinal cord of injured mice
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 organ: Spinal cord selection marker: Time following spinal cord injury treatment: spinal cord injury time: 7 days
|
Treatment protocol |
The spinal cords of deeply anesthetized mice were exposed by laminectomy at T12, and contusive (200 kdynes) centralized injury was performed using the Infinite Horizon spinal cord impactor (Precision Systems)
|
Growth protocol |
In vivo-sorted cells
|
Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy (Qiagen) see Jaitin et. al., Science 2014
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
INVIVO.xlsx
|
Data processing |
Illumina Casava1.8 software used for basecalling. alignment: tophat with deafult parameters Expression table done using ESAT http://garberlab.umassmed.edu/software/esat/ Genome_build: mm9 Supplementary_files_format_and_content: tab-delimited text files include mRNA molecule count values for each Sample
|
|
|
Submission date |
Oct 24, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Ido Amit |
E-mail(s) |
ido.amit@weizmann.ac.il
|
Phone |
972-8-9343338
|
Organization name |
Weizmann Institute of Science
|
Department |
Immunology
|
Street address |
234 Herzl st.
|
City |
Rehovot |
ZIP/Postal code |
760001 |
Country |
Israel |
|
|
Platform ID |
GPL18480 |
Series (1) |
GSE62698 |
Spinal cord injury (RNA sequencing data) |
|
Relations |
BioSample |
SAMN03142888 |
SRA |
SRX742750 |