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Sample GSM1531574 Query DataSets for GSM1531574
Status Public on Dec 03, 2014
Title mic_1hr-1
Sample type SRA
 
Source name Microglia sorted from spinal cord of injured mice
Organism Mus musculus
Characteristics strain: C57BL/6
organ: Spinal cord
selection marker: Time following spinal cord injury
treatment: spinal cord injury
time: 1 hr
Treatment protocol The spinal cords of deeply anesthetized mice were exposed by laminectomy at T12, and contusive (200 kdynes) centralized injury was performed using the Infinite Horizon spinal cord impactor (Precision Systems)
Growth protocol In vivo-sorted cells
Extracted molecule total RNA
Extraction protocol miRNeasy (Qiagen)
see Jaitin et. al., Science 2014
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description INVIVO.xlsx
Data processing Illumina Casava1.8 software used for basecalling.
alignment: tophat with deafult parameters
Expression table done using ESAT http://garberlab.umassmed.edu/software/esat/
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include mRNA molecule count values for each Sample
 
Submission date Oct 24, 2014
Last update date May 15, 2019
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platform ID GPL18480
Series (1)
GSE62698 Spinal cord injury (RNA sequencing data)
Relations
BioSample SAMN03142874
SRA SRX742733

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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