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Sample GSM1531571 Query DataSets for GSM1531571
Status Public on Dec 03, 2014
Title b04_WT_Irf7_t4
Sample type SRA
Source name GM-CSF treated myeloid cells
Organism Mus musculus
Characteristics cell type: Myeloid cells
tissue: Bone marrow
strain: C57BL/6
chip antibody: Irf7
Treatment protocol LPS treatment 2hr
Growth protocol GM-CSF supplemented culture
Extracted molecule genomic DNA
Extraction protocol Cells were fixated, lyzed and nuclei were sonicated. Sonicated nuclei were mixed with protein G magnetic dynabeads coupled to target antibbody.
Libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 1500
Data processing Basecalls performed using CASAVA version 1.4
alignment using bowtie 0.12 using default parameters
tdf command - igvtools count -w 5 -e 200
Genome_build: mm9
Supplementary_files_format_and_content: tdf files
Submission date Oct 24, 2014
Last update date May 15, 2019
Contact name Ido Amit
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
Platform ID GPL18480
Series (1)
GSE62697 ChIP-seq for IRF7
BioSample SAMN03142847
SRA SRX742698

Supplementary file Size Download File type/resource
GSM1531571_b04_WT_Irf7_t4.tdf 230.6 Mb (ftp)(http) TDF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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