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Sample GSM1531197 Query DataSets for GSM1531197
Status Public on Dec 04, 2014
Title Sperm of high sugar fed male flies - Replicate 2
Sample type SRA
 
Source name Sperm
Organism Drosophila melanogaster
Characteristics strain: w1118
developmental stage: Adult male
tissue: Spermatozoa
treatment: 300g/L of sugar
Extracted molecule total RNA
Extraction protocol Purified total RNA, extracted with Trizol and treated with Ribo-ZeroTM rRNA removal kit from epicenter.
TruSeq® stranded sample preparation kit from Illumina.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Description Purified total RNA was treated with Ribo-ZeroTM rRNA removal kit from epicenter.
Data processing Reads were mapped using TopHat (Kim et al, 2013) v2.0.8 with -G option.
Gene expression values and significantly differentially expressed genes were calculated using Cuffdiff (Trapnell et al, 2012) v2.1.1 with upper quartile normalization and weighting multi-mapping reads (-N -u options).
Genome_build: BDGP5
Supplementary_files_format_and_content: Tab delimited text files. Gene expression values and statistical significance of differential expression calculated by CuffDiff.
 
Submission date Oct 23, 2014
Last update date May 15, 2019
Contact name Tanya Vavouri
Organization name Josep Carreras Leukemia Research Institute
Street address Ctra de Can Ruti, Cami de les Escoles, s/n
City Badalona
State/province BARCELONA
ZIP/Postal code 08019
Country Spain
 
Platform ID GPL16479
Series (1)
GSE62668 Paternal diet defines offspring chromatin state and intergenerational obesity.
Relations
Reanalyzed by GSM3287516
BioSample SAMN03140394
SRA SRX739985

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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