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Sample GSM1529538 Query DataSets for GSM1529538
Status Public on Oct 07, 2019
Title Sample 576_Treg_HPGD_KO
Sample type SRA
Source name Treg_HPGD_KO
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: HPGD KO
tissue: spleen
cell type: regulatory CD4+ T cells
Growth protocol Cells were isolated from spleens
Extracted molecule total RNA
Extraction protocol Cells were isolated by cell sorting, lyzed in Qiazol and RNA isolated. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 10 ng of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description Sample 576
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using TopHat with default parameters.
mRNA quantification according to RefSeq Transcripts - 2014-07-30.
raw read counts were normalized by DE-Seq.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include DE-Seq normailzed expression values for each Sample.
Submission date Oct 22, 2014
Last update date Oct 07, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platform ID GPL18480
Series (1)
GSE62579 Prostaglandin E2-mediated T cell suppression requires expression of hydroxyprostaglandin dehydrogenase in regulatory T cells.
BioSample SAMN03135123
SRA SRX738126

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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