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Sample GSM1527856 Query DataSets for GSM1527856
Status Public on Oct 21, 2014
Title mouse lung infected 2 days, biological rep1
Sample type RNA
 
Source name mouse lung infected 2 days with PR8 H1N1 influenza virus
Organism Mus musculus
Characteristics tissue: lung
strain: ICR
Treatment protocol Whole mouse lung tissues were homogenized in liquid nitrogen. Total RNAs were extracted from mouse lungs using QIAGEN RNeasy Mini Kit.
Growth protocol Mice were anesthetized with ether and were intranasally inoculated with 104 TCID50 of PR8 virus in physiological saline.For the total RNAs extraction, entire lungs from mice infected by PR8 virus were randomly selected and harvested on the 2th and 5th day.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted from mouse lungs using QIAGEN RNeasy Mini Kit,according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description 13~15g
microRNA expression data
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 20, 2014
Last update date Oct 21, 2014
Contact name yanyan bao
E-mail(s) baoyanyan885@126.com
Organization name Biosafety Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
Street address Dongzhimen South Street
City beijing
ZIP/Postal code 100700
Country China
 
Platform ID GPL19117
Series (1)
GSE62495 MicroRNA expression data from H1N1 influenza virus infected mouse lung

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
MIMAT0000121_st 12.33616
MIMAT0004519_st 3.668988
MIMAT0000122_st 13.97413
MIMAT0004520_st 5.804455
MIMAT0016979_st 0.8557289
MIMAT0000123_st 6.257287
MIMAT0000124_st 12.64587
MIMAT0004521_st 0.6128161
MIMAT0016980_st 5.623018
MIMAT0000125_st 14.65197
MIMAT0004522_st 8.032629
MIMAT0000126_st 13.21854
MIMAT0004523_st 6.180564
MIMAT0000127_st 5.769789
MIMAT0000128_st 13.60292
MIMAT0000129_st 10.94403
MIMAT0000130_st 12.96149
MIMAT0004524_st 7.532223
MIMAT0000131_st 13.21817
MIMAT0016981_st 1.30342

Total number of rows: 3222

Table truncated, full table size 75 Kbytes.




Supplementary file Size Download File type/resource
GSM1527856_2day-1_miRNA-4_0_.CEL.gz 832.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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