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Sample GSM1527752 Query DataSets for GSM1527752
Status Public on Jun 08, 2016
Title Donor2 day 13 Th2 unactivated
Sample type RNA
 
Channel 1
Source name Th2
Organism Homo sapiens
Characteristics cell type: Th2
activation: Donor2 day 13 Th2 unactivated
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trisure following manufacturer's instructions
Label Cy3
Label protocol Sample RNA was mixed with spike-in control (Agilent, # 5188-5279) A and labelled with Cy3-CTP. Reference RNA (Stratagene Universal Human Reference RNA, Agilent) was mixed with spike-in control B and labelled with Cy5-CTP.
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics sample type: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trisure following manufacturer's instructions
Label Cy5
Label protocol Sample RNA was mixed with spike-in control (Agilent, # 5188-5279) A and labelled with Cy3-CTP. Reference RNA (Stratagene Universal Human Reference RNA, Agilent) was mixed with spike-in control B and labelled with Cy5-CTP.
 
 
Hybridization protocol Sample cRNA was mixed with reference cRNA and hybridised to SurePrint G3 Human Gene Expression 8x60K microarrays . Hybridisation and washing of the array slides was done following the specifications in the Agilent Low Input Quick Amp Labeling protocol.
Scan protocol Arrays were scanned on an Agilent High Resolution C scanner and images were quantified using Agilent Feature Extraction Software (version 10.7)
Data processing Fluorescence intensities were converted into log base 10 expression ratio of Cy5 to Cy3 using Feature Extraction software
Data was converted into log base 2 expression ratios of Cy3 (sample) to Cy5 (reference) signal.
 
Submission date Oct 18, 2014
Last update date Jun 08, 2016
Contact name Richard Jenner
E-mail(s) r.jenner@ucl.ac.uk
Organization name UCL Cancer Institute
Department Cancer Biology
Lab Regulatory Genomics
Street address 72 Huntley Street
City London
ZIP/Postal code WC1E 6BT
Country United Kingdom
 
Platform ID GPL13607
Series (2)
GSE62484 T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation (Agilent 1)
GSE62486 T-bet recruits P-TEFb to super-enhancers to regulate T helper cell differentiation

Data table header descriptions
ID_REF
VALUE Median centered, log2 ratio (test/reference)

Data table
ID_REF VALUE
4 2.597806202
5 -0.346727325
6 0.638715722
7 -1.487988063
8 -0.386843936
9 1.990972548
10 0.609720048
11 0
12 0.952721074
13 0.00023571
14 -0.65481636
15 0.124408958
16 -1.422242789
17 1.213656099
18 -0.964866125
19 -0.610869162
20 -0.457719116
21 0.484447422
24 0.81716485
25 -1.395936223

Total number of rows: 58717

Table truncated, full table size 924 Kbytes.




Supplementary file Size Download File type/resource
GSM1527752_Donor2_d13_Th2_unactivated.txt.gz 6.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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