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Sample GSM1526688 Query DataSets for GSM1526688
Status Public on Sep 01, 2018
Title LNCaP, replicate3
Sample type RNA
 
Source name LNCaP
Organism Homo sapiens
Characteristics cell type: androgen-sensitive human prostate adenocarcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Trizol.
Label SYBR Green
Label protocol cDNA was synthesised from 1ug total RNA using Superscript 3 Reverse Transcriptase (Life Technologies) and Quantitative real-time PCR performed using an Applied Biosciences 7900HT following the Manufacturer’s instructions. Primers were obtained from Integrated DNA Technologies.
 
Hybridization protocol N/A
Scan protocol N/A
Description Cancer
LNCaP.3
Data processing Mean target gene signals were normalised to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The 'averaged_normalized.txt' reports normalised mean signal (against housekeeping gene ALAS1).
All deltadeltaCt based fold-change calculations were performed in the R statistical package.
the 'fold_change.txt' reports test/control (i.e., LNCaP/PrEC) ratios.
Please note that sample data table contains raw Ct values
 
Submission date Oct 16, 2014
Last update date Sep 01, 2018
Contact name Timothy John Peters
E-mail(s) t.peters@garvan.org.au
Phone +61292958325
Organization name Garvan Institute of Medical Research
Department Immunology
Lab Immunogenomics
Street address 384 Victoria St
City Darlinghurst
State/province NSW
ZIP/Postal code 2010
Country Australia
 
Platform ID GPL19299
Series (2)
GSE62409 Real-time quantitative PCR analysis of Normal and Cancerous Prostate Cells [RT-PCR]
GSE62410 Transcriptome profiling of Normal and Cancerous Prostate Cells

Data table header descriptions
ID_REF
VALUE raw Ct value

Data table
ID_REF VALUE
1 24.925898
2 22.377214
3 32.57435
4 30.15494
5 23.2199
6 31.060421
7 28.618845
8 34.705654
9 37.32221
10 28.075665
11 23.846998
12
13 27.038502
14 31.173553
15 31.695799
16 32.147457
17 31.969755
18 28.467424
19 27.800749
20 26.12416

Total number of rows: 40

Table truncated, full table size <1 Kbytes.




Supplementary data files not provided
Processed data provided as supplementary file

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