|
| Status |
Public on May 21, 2015 |
| Title |
daf-16(mgDF47) larva at 15, prep2 |
| Sample type |
RNA |
| |
|
| Source name |
C.elegans daf-16(mgDF47) mutant larvae at 15 degree
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: daf-16(mgDF47)
age: Late L4
treatment: 15 C
|
| Treatment protocol |
worms are growing from embryo to late stage L4 worms or aging from late L4 worms to Day3 old adult at 15 and 25 degree
|
| Growth protocol |
worms are growing and ageing on normal NGM plates at different temperature temporally
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs are extracted with TRI reagent(Life Technoligies)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard affymetrix protocol at the Microarray Core Facility in University of Michigan
|
| |
|
| Hybridization protocol |
Total RNA was used for hybridization with Affymetrix C.elegans Gene 1.1 ST array strip at the Microarray Core Facility in University of Michigan
|
| Scan protocol |
Follow standard Affymetrix protocol
|
| Description |
gene expression data from Late L4 stage daf-16(mgDF47) worms at 15 degree
|
| Data processing |
gene expression values are processed using RMA methods with R software.
|
| |
|
| Submission date |
Oct 14, 2014 |
| Last update date |
May 21, 2015 |
| Contact name |
X.Z Shawn Xu |
| E-mail(s) |
shawnxu@umich.edu
|
| Phone |
734-615-9311
|
| Organization name |
Life Sciences Institute, University of Michigan
|
| Street address |
210 Washtenaw Ave, Room 6115A
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19291 |
| Series (1) |
| GSE62297 |
Whole-genome expression profiling of C. elegans larvae and adults |
|
