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Sample GSM152360 Query DataSets for GSM152360
Status Public on Mar 10, 2007
Title 733T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 49, Histology: IDC + ILC, Tumor stage: 1, Tumor grade: 2
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Mar 09, 2007
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 455.1 P 0.001602
1053_at 93.7 P 0.007543
117_at 102.4 P 0.000959
121_at 321.7 P 0.014937
1255_g_at 23 A 0.107301
1294_at 76.7 P 0.000805
1316_at 34 P 0.02786
1320_at 9.8 A 0.715253
1405_i_at 137.7 P 0.002607
1431_at 17.7 A 0.127645
1438_at 41.7 A 0.438361
1487_at 116.5 M 0.060419
1494_f_at 85.6 P 0.024711
1598_g_at 380.5 P 0.001354
160020_at 148.4 P 0.013092
1729_at 127.3 P 0.006532
177_at 40.9 P 0.031336
1773_at 41.6 A 0.150527
179_at 154.7 P 0.048995
1861_at 35.6 A 0.11716

Total number of rows: 22283

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM152360.CEL.gz 3.6 Mb (ftp)(http) CEL

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