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Sample GSM152356 Query DataSets for GSM152356
Status Public on Mar 10, 2007
Title 719T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 45, Histology: IDC, Tumor stage: 1, Tumor grade: 1
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Mar 09, 2007
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 488.3 P 0.002228
1053_at 54 P 0.006532
117_at 42.4 A 0.127645
121_at 329.8 P 0.007543
1255_g_at 16.8 A 0.175989
1294_at 60.9 P 0.031336
1316_at 39.7 P 0.021866
1320_at 12.2 A 0.660442
1405_i_at 38.5 P 0.020663
1431_at 16.1 A 0.175989
1438_at 37.8 A 0.378184
1487_at 138.6 P 0.043968
1494_f_at 93.2 P 0.021866
1598_g_at 409.9 P 0.001602
160020_at 211.6 P 0.021866
1729_at 85.1 P 0.035163
177_at 55.1 M 0.05447
1773_at 32.1 A 0.07383
179_at 120.9 P 0.02786
1861_at 51 A 0.162935

Total number of rows: 22283

Table truncated, full table size 583 Kbytes.

Supplementary file Size Download File type/resource
GSM152356.CEL.gz 3.6 Mb (ftp)(http) CEL

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