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Sample GSM152352 Query DataSets for GSM152352
Status Public on Mar 10, 2007
Title 496T
Sample type RNA
 
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 65, Histology: IDC, Tumor stage: 2, Tumor grade: 3
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
 
Submission date Dec 21, 2006
Last update date Sep 23, 2011
Contact name Andreas Klein
E-mail(s) andreas.klein@charite.de
Phone +49 30 450 528087
Fax +49 30 450 52945
URL http://www.charite.de/molbiol/bioinf/tumbiol/
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers
Relations
Reanalyzed by GSE31519

Data table header descriptions
ID_REF GPL96
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 636.5 P 0.003067
1053_at 34.8 A 0.138765
117_at 165.5 P 0.004863
121_at 454.2 P 0.011447
1255_g_at 22.6 A 0.52062
1294_at 225.4 P 0.000959
1316_at 43.4 P 0.013092
1320_at 5 A 0.621816
1405_i_at 269.3 P 0.001165
1431_at 22.4 A 0.127645
1438_at 22 A 0.64131
1487_at 278.5 A 0.089405
1494_f_at 90.6 P 0.039365
1598_g_at 303.9 M 0.05447
160020_at 283.5 P 0.024711
1729_at 150.5 A 0.089405
177_at 53.6 P 0.021866
1773_at 51.1 A 0.47938
179_at 152.4 A 0.107301
1861_at 19.2 A 0.541184

Total number of rows: 22283

Table truncated, full table size 585 Kbytes.




Supplementary file Size Download File type/resource
GSM152352.CEL.gz 3.6 Mb (ftp)(http) CEL

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