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Sample GSM152348 Query DataSets for GSM152348
Status Public on Mar 10, 2007
Title 303T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 57, Histology: IDC, Tumor stage: 1, Tumor grade: 2
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Mar 09, 2007
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 760.5 P 0.001892
1053_at 49.2 A 0.138765
117_at 58.7 A 0.339558
121_at 273 P 0.039365
1255_g_at 31.6 A 0.150527
1294_at 137.7 P 0.001892
1316_at 79.7 P 0.003585
1320_at 22.5 A 0.581931
1405_i_at 25.2 A 0.562354
1431_at 13 A 0.234557
1438_at 44.3 A 0.35869
1487_at 176.6 A 0.07383
1494_f_at 85.1 P 0.048995
1598_g_at 731.6 P 0.000959
160020_at 441.4 P 0.009985
1729_at 138.2 P 0.021866
177_at 32.6 A 0.175989
1773_at 53.3 A 0.175989
179_at 159.2 P 0.024711
1861_at 58.1 A 0.397994

Total number of rows: 22283

Table truncated, full table size 585 Kbytes.

Supplementary file Size Download File type/resource
GSM152348.CEL.gz 3.5 Mb (ftp)(http) CEL

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