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Sample GSM152345 Query DataSets for GSM152345
Status Public on Mar 10, 2007
Title 263T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 75, Histology: ILC, Tumor stage: 4, Tumor grade: 2
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Mar 09, 2007
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 635.5 P 0.000388
1053_at 75.1 M 0.05447
117_at 64.1 P 0.000673
121_at 269 P 0.017001
1255_g_at 35.3 P 0.048995
1294_at 150.6 P 0.000219
1316_at 50.4 P 0.006532
1320_at 29.4 A 0.438361
1405_i_at 49.6 P 0.002143
1431_at 20.4 A 0.138765
1438_at 18.4 A 0.660442
1487_at 141.7 P 0.035163
1494_f_at 88.6 P 0.017001
1598_g_at 515.5 P 0.000562
160020_at 234.3 P 0.017001
1729_at 131.5 P 0.003067
177_at 46.4 P 0.019304
1773_at 55 A 0.218983
179_at 150.7 P 0.035163
1861_at 48.8 A 0.162935

Total number of rows: 22283

Table truncated, full table size 585 Kbytes.

Supplementary file Size Download File type/resource
GSM152345.CEL.gz 3.7 Mb (ftp)(http) CEL

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