GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM152344 Query DataSets for GSM152344
Status Public on Mar 10, 2007
Title 218T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 29, Histology: IDC, Tumor stage: 2, Tumor grade: 2
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Sep 23, 2011
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers
Reanalyzed by GSE31519

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 397.2 P 0.001892
1053_at 131.9 P 0.000805
117_at 17.8 A 0.07383
121_at 279.7 P 0.005643
1255_g_at 38.3 A 0.098054
1294_at 89.2 P 0.039365
1316_at 48.1 P 0.017001
1320_at 7.2 A 0.660442
1405_i_at 44 P 0.020663
1431_at 12.6 A 0.32083
1438_at 23 A 0.47938
1487_at 144.4 P 0.048995
1494_f_at 70.7 P 0.031336
1598_g_at 197.8 P 0.014937
160020_at 206 P 0.017001
1729_at 187.3 P 0.001354
177_at 43.3 P 0.039365
1773_at 34.7 A 0.5
179_at 151.9 A 0.066865
1861_at 60.3 A 0.089405

Total number of rows: 22283

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM152344.CEL.gz 3.8 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap