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Sample GSM152343 Query DataSets for GSM152343
Status Public on Mar 10, 2007
Title 210T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 85, Histology: IDC, Tumor stage: 4, Tumor grade: 3
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Sep 23, 2011
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers
Reanalyzed by GSE31519

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 630.6 P 0.000959
1053_at 200.4 P 0.000562
117_at 41.3 M 0.060419
121_at 325.2 P 0.008689
1255_g_at 16.9 A 0.150527
1294_at 83.5 P 0.017001
1316_at 58.6 P 0.005643
1320_at 25.3 A 0.541184
1405_i_at 274.9 P 0.000762
1431_at 23 A 0.150527
1438_at 42.3 A 0.397994
1487_at 244.9 A 0.07383
1494_f_at 98.2 P 0.021866
1598_g_at 339.5 P 0.003585
160020_at 146 P 0.019304
1729_at 189.6 P 0.035163
177_at 36.4 A 0.107301
1773_at 41.8 A 0.458816
179_at 187.4 P 0.043968
1861_at 52.6 A 0.175989

Total number of rows: 22283

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM152343.CEL.gz 3.7 Mb (ftp)(http) CEL

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